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By W.E. Gutteridge

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Invadens is, however, specific to NADH. Alternatively, pyruvate is converted via acetyl CoA and acetyl phosphate to acetate. The mechanism of these conversions seems to depend on the environmental conditions. In the presence of oxygen, the enzyme pyruvate oxidase (CoA acetylating) is active pyruvate + CoA + 0 2 ~ acetyl CoA + C0 2 + H 20 2 FAD apparently is required, although its exact role is uncertain. 6). Iron-sulphur proteins are present in Entamoeba and probably serve as the electron transport protein CATABOLISM.

Brucei T. evansi T. equinum T. equiperdum T. vivax T. congolense T. lewisi 0 0... + ++ ++ 0 u ;;... J + + + + + + tfl ++ ++ ++ ++ ++ ++ ± ± ± + + + + + + + + -,none;±, trace;+, small quantities;++, substantial quantities members of the subgenus Trypanozoon, of which Trypanosoma brucei is the most studied, produce mainly pyruvate. A second group, containing the South American trypanosomes of which T. cruzi is the best understood, produce mainly C0 2 but also some acetate and succinate. The third group, containing the African trypanosomes T.

1 Energy stores Glycogen normally is present, distributed throughout the cell and is responsible for 10-30 per cent of its dlj' weight. Enzymes similar to those present in mammalian cells catalyse the synthesis and catabolism of the glycogen. 2 Substrate utnisation Trichomonads will utilise hexoses, disaccharides and polysaccharides with a( 1 - 4) glycosidic linkages. Although maltose supports growth best, glucose is used most frequently in laboratory culture media. Amylases are present in trichomonads.

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