By Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji Ikura, Masaya Nagao, Akira Ichikawa, Kiichiro Teruya (eds.)
Animal mobile expertise is a turning out to be self-discipline of mobile biology which goals not just to appreciate constructions, features and behaviors of differentiated animal cells, but in addition to check their talents for use for business and scientific reasons. The objective of animal telephone know-how contains the clonal growth of differentiated cells, the optimization in their tradition stipulations, modulation in their skill to provide proteins of clinical and pharmaceutical importantance, and the appliance of animal cells to gene remedy, synthetic organs and the creation of sensible meals. This quantity supplies the readers an entire evaluate of the current cutting-edge and should be important for these operating in both educational environments or within the biotechnology and pharmaceutical sectors, really mobile biologists, biochemists, molecular biologists, immunologists, biochemical engineers and all different disciplines relating to animal mobile culture.
Read or Download Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006 PDF
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Extra info for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006
However, even by using this method, it is laborious and time-consuming task to establish highly productive cell lines. These backgrounds promoted us to develop an oncogene-activated production (OAP) system which enables rapid establishment of recombinant protein hyper-producing cell lines. The OAP system is constructed by introducing a reporter plasmid carrying a gene of interest and effector plasmids carrying oncogenes into host cells. Previously, we have optimized the combination between oncogenes and promoters used in the OAP system.
2 Proteome Analysis Using 2-DE To investigate the protein profiles of 2E3-O cells treated with sericin, 2-DE was performed. The results are shown in Fig. 2. Several distinct spots were identified Fig. 1 Mitogenic effect of sericin at various concentrations. 15%). 15% 40 Fig. 2 (a) 2-D protein profile of the 2E3-O cell line. The vertical axis indicates the molecular weight, while the horizontal axis indicates the isoelectric point. (b) Differential spots between the samples from cells treated with sericin and those without T.
Keywords Cryopreservation • hybridoma • insulinoma • sericin • serum-free 1 Introduction Mammalian cell culture is applicable to many fields. Various biological substances are produced as medicines by cultured mammalian cells, because of their high bioactivity due to glycosylation. In cell culture, the risk of contamination by bacteria, fungi, mycoplasmas and viruses is always a concern. In addition, cell function is impaired gradually during the culture period. During cryopreservation, cells arrest their metabolic pathways, therefore making it possible to maintain their functions.